B73 RefGen_v4cen - Manual Reassembly of the 106Mb of centromeric and pericentromeric sequence RefGen_v4 genome assembly.

Reassemblies of the centromeric regions of Zea mays B73 constructed from existing, and publicly available PacBio read data generated for the B73 RefGen_v4 sequencing project, presented in Jiao et al. 2017 (Bioproject PRJNA10769, Pac Bio SRA Accession SRX1472849). Gaps in the centromeres and pericentromeres of the RefGen_v4 assembly were filled with previously unincorporated RefGen_v4 contigs or reads using unique junctions and the RefGen_v2 BAC by BAC assembly (NCBI Assembly Accession GCA_000005005.4) to walk across gaps. Assembly was assisted by Junction Viewer annotation software (Wolfgruber et al. 2010) and final sequence assembly using Geneious 6.0 software.

Data and Resources

Additional Info

Field Value
Author Daniel Laspisa, Kevin Schneider, Gernot Presting
Last Updated June 25, 2024, 19:56 (UTC)
Created June 23, 2024, 17:05 (UTC)
Citation Daniel Laspisa, Kevin Schneider, Gernot Presting 2019. B73 RefGen_v4cen - Manual Reassembly of the 106Mb of centromeric and pericentromeric sequence RefGen_v4 genome assembly.. CyVerse Data Commons. DOI 10.25739/7y1p-5169
Contributor Presting Laboratory, University of Hawaii
Date 2019
Date created in discovery environment 2019-01-30 19:40:32
Date last modified in discovery environment 2020-02-20 22:05:33
Description Reassemblies of the centromeric regions of Zea mays B73 constructed from existing, and publicly available PacBio read data generated for the B73 RefGen_v4 sequencing project, presented in Jiao et al. 2017 (Bioproject PRJNA10769, Pac Bio SRA Accession SRX1472849). Gaps in the centromeres and pericentromeres of the RefGen_v4 assembly were filled with previously unincorporated RefGen_v4 contigs or reads using unique junctions and the RefGen_v2 BAC by BAC assembly (NCBI Assembly Accession GCA_000005005.4) to walk across gaps. Assembly was assisted by Junction Viewer annotation software (Wolfgruber et al. 2010) and final sequence assembly using Geneious 6.0 software.
New Attribute New Value
Publisher CyVerse Data Commons
Rights No restrictions for the use of these data following publication.
Source Jiao et al. 2017 (Bioproject PRJNA10769, Pac Bio SRA Accession SRX1472849)
assembly_method Manual
assembly_name B73_RefGen_v4CEN
contact_email gernot@hawaii.edu, dlaspisa@hawaii.edu
contact_first_name Gernot, Daniel
contact_last_name Presting, Laspisa
final_version Yes
genome_full Yes
genome_subset Centromeres and Pericentromeres
identifierType DOI
organization_address_city Honolulu
organization_address_country United States
organization_address_department Molecular Bioscience and Bioengineering
organization_address_institution University of Hawaii
organization_address_postal_code 96822
organization_address_street 1955 East West Rd. Agricultural Sciences 218
publisher CyVerse Data Commons
resourceType Centromere and pericentromere sequence assemblies
sequencing_technology PacBio
wgs_submission_type the wgs contigs and any other components of the AGP file are already in GenBank